comparative proteomics analysis of two strains of neisseria meningitidis serogroup b and neisseria lactamica
Authors
abstract
conclusions these results show the usefulness of proteome analysis in successful identification of the common proteins between n. lactamica strains and nmb. this proteomics analysis is the starting point in the path of knowledge development about whole proteome profiles of n. lactamica strains. objectives the main aims of this study were to determine whole proteome profiles of n. lactamica strains and to compare them with whole proteome profile of a reference strain of nmb for identification of some of common proteins between the two species. materials and methods we compared the whole proteomic profiles of n. lactamica strains and a reference strain of nmb. lysates from bacterial strains were resolved by two-dimensional gel electrophoresis (2-de), followed by coomassie brilliant blue staining. some of the protein spots were excised from the gel and subjected to matrix-assisted laser desorption/ionization-tandem time-of-flight mass spectrometry (maldi-tof/tof ms) analysis. results the analysis of coomassie-stained gels using imagemaster 2d platinum software identified approximately 800 reproducible protein spots in the range of pi 4.5 - 9.5 and mr of 8 - 100 kda for each 2-de gel of the studied bacterial strains. by comparing proteome maps of 2-de gels, more than 200 common protein spots were recognized between the two species. forty-eight common protein spots between the studied bacterial strains were identified by maldi-tof/tof-ms. the results indicated that among the protein spots identified by moldi-tof/tof mass spectrometry, the groups of proteins included cell surface, energy metabolism, amino acid transport and metabolism, coenzyme metabolism, defense, multifunctional cellular processes, dna, rna and protein synthesis, ribosomal structure, regulatory functions, replication, transcription, translation, unknown and hypothetical proteins with unknown function. we found that n. lactamica strains have a proteome profile somewhat similar to each other and slightly different with nmb. background antigenic similarities between neisseria lactamica as a commensal species and n. meningitidis serogroup b (nmb) as an important cause of meningitis infection have been considered for the development of an effective vaccine based on their common proteins to prevent life-threatening bacterial meningitis.
similar resources
Comparative Proteomics Analysis of Two Strains of Neisseria meningitidis Serogroup B and Neisseria lactamica
BACKGROUND Antigenic similarities between Neisseria lactamica as a commensal species and N. meningitidis serogroup B (NmB) as an important cause of meningitis infection have been considered for the development of an effective vaccine based on their common proteins to prevent life-threatening bacterial meningitis. OBJECTIVES The main aims of this study were to determine whole proteome profiles...
full textIn silico Analysis and Modeling of ACP-MIP–PilQ Chimeric Antigen from Neisseria meningitidis Serogroup B
Background: Neisseria meningitidis, a life-threatening human pathogen with the potential to cause large epidemics, can be isolated from the nasopharynx of 5–15% of adults. The aim of the current study was to evaluate biophysical and biochemical properties and immunological aspects of chimeric acyl-carrier protein-macrophage infectivity potentiator protein-type IV pilus biogenesis protein ...
full textGenetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains.
We compared the results obtained by serotyping of PorB epitopes using an expanded panel of monoclonal antibodies (mAb) including mAb 7 and mAb 10, with results obtained by RFLP of rRNA genes (ribotyping). The purpose of this study was to assess the correlation between phenotypic- and genotypic- methods for typing N. meningitidis. The ribotypes obtained using ClaI or EcoRV endonucleases grouped ...
full textDevelopment of a DNA Aptamer for Screening Neisseria meningitidis Serogroup B by Cell SELEX
Background: Artificial oligonucleotides like DNA or RNA aptamers can be used as biodiagnostic alternatives for antibodies to detect pathogens. Comparing to antibodies, artificial oligonucleotides are produced easily at lower costs and are more stable. Neisseria meningitidis, the causative agent of meningitis, is responsible for about 1% of infections in an epidemic period. Specific DNA aptamers...
full textHomology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica.
DNA probe hybridisation was used to examine the relation between the cryptic plasmid from Neisseria gonorrhoeae and plasmids carried by pharyngeal isolates of Neisseria meningitidis and Neisseria lactamica. The complete gonococcal cryptic plasmid and HinfI derived digestion fragments subcloned into Escherichia coli were used to probe Southern blots of plasmid extracts. Homology was found to a p...
full textNeisseria meningitidis Serogroup W135, China
Technical Appendix Figure 1. Distribution and molecular characterization of Neisseria meningitidis W135 isolates from 3 patients, People's Republic of China, and from Taiwan. ST, sequence type. Figure 2. Distribution and molecular characterization of Neisseria meningitidis W135 isolates from case-patients, People's Republic of China. Minimum spanning tree of 12 W135 isolates typed by multilocus
full textMy Resources
Save resource for easier access later
Journal title:
jundishapur journal of microbiologyجلد ۸، شماره ۱۱، صفحات ۰-۰
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023